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This document is applicable to fertilizing products, which are classified as PFC1(A) and PFC1(B) or the PFC1(A) and PFC1(B) component in PFC7 of Regulation (EU)2019/1009[4]. However, the present method was not validated for blends.
This document specifies a method for the determination of the biuret content by high-performance liquid chromatography (HPLC) with UV detector. The method is applicable to organic and organo-mineral fertilizers containing urea.
Nowadays, there is a method standardized as EN15479 that allows the determination of biuret in urea by spectrophotometric detection. Organic and organo-mineral fertilizers contain organic matter and other compounds apart from urea that would interfere in a spectrophotometric method. HPLC allows an accurate determination of biuret by separating it from possible interfering compounds [2] [3].
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